The Registry of Toxic Effects of Chemical Substances

o - Phenylenediamine, 4 - nitro -

RTECS #: ST2975000

CAS #: 99-56-9

UPDATE: November 2004 MW: 153.16 MF: C6H7N3O2


NOTE:

TABLE OF CONTENTS:
  1. SYNONYMS:
  2. MUTATION DATA:
  3. REPRODUCTIVE EFFECTS DATA:
  4. ACUTE TOXICITY DATA:
  5. REVIEWS:
  6. NIOSH DOCUMENTATION AND SURVEILLANCE:
  7. STATUS IN FEDERAL AGENCIES:
  8. REFERENCES:

SYNONYMS:

  1. 1,2 - Diamino - 4 - nitrobenzene
  2. 2 - Amino - 4 - nitroaniline
  3. 3,4 - Diaminonitrobenzene
  4. 4 - N - o - Pda
  5. 4 - NO
  6. 4 - Nitro - 1,2 - benzenediamine
  7. 4 - Nitro - 1,2 - diaminobenzene
  8. 4 - Nitro - 1,2 - fenylendiamin (Czech)
  9. 4 - Nitro - 1,2 - phenylenediamine
  1. 4 - Nitro - o - phenylene - diamine
  2. 4 - Nitro - o - phenylenediamine
  3. 4 - Nop
  4. 4 - Nopd
  5. 4NDB
  6. C.I. 76020
  7. NCI - C03941
  8. NOPD
  9. p - Nitro - o - phenylenediamine


SKIN AND EYE IRRITATION DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
N/R
 N/R N/R N/R


MUTATION DATA AND REFERENCES:

SYSTEM TEST ROUTE/
ORGANISM/
TISSUE 		DOSE REFERENCE
cytogenetic analysis hamster ovary 138 mg/L EMMUEG 27,67,1996
cytogenetic analysis hamster fibroblast 25 mg/L MUREAV 40,47,1976
cytogenetic analysis hamster other cell types 10 µmol/L/24 hour NATUAS 260,368,1976
cytogenetic analysis hamster other cell types 10 µmol/L NATUAS 260,368,1976
cytogenetic analysis hamster lung 40 mg/L GMCRDC 27,95,1981
dominant lethal test intraperitoneal
mouse 		3,000 mg/kg/10 week- intermittent SheCW# 25MAR1977
DNA damage mouse lymphocyte 104 µmol/L MUREAV 203,155,1988
DNA repair Escherichia coli 10 mg/L CRNGDP 2,189,1981
unscheduled DNA synthesis human HeLa cell 1 mmol/L CNREA8 38,2621,1978
unscheduled DNA synthesis rat liver 500 µg/L EMMUEG 26,147,1995
mutation in microorganisms Aspergillus nidulans 200 mg/L (-enzymatic activation step) MUREAV 97,293,1982
mutation in microorganisms Bacillus subtilis 50 mg/L (-enzymatic activation step) MUREAV 95,191,1982
mutation in microorganisms Escherichia coli 0.25 mg/plate/90 minute (-enzymatic activation step) MUREAV 535,55,2003
mutation in microorganisms mouse lymphocyte 13,100 µg/L (+enzymatic activation step) EMMUEG 12(Suppl 13),37,1988
mutation in microorganisms Salmonella typhimurium 1 µg/plate (+/-enzymatic activation step) ENMUDM 8(Suppl 7),1,1986
mutation in microorganisms Salmonella typhimurium 10 µg/plate (-enzymatic activation step) JAPTO* 20,491,2000
mutation in microorganisms Salmonella typhimurium 0.1 mg/plate/48 hour (-enzymatic activation step) JAPTO* 22,45,2001
mutation in microorganisms Salmonella typhimurium 0.1 mg/plate/20 minute (-enzymatic activation step) EMMUEG 36,13,2000
mutation in microorganisms Salmonella typhimurium 2 µL/plate (-enzymatic activation step) VHTODE 42,77,2000
mutation in microorganisms Salmonella typhimurium 0.2 mg/plate/45 minute (-enzymatic activation step) TCMUD8 22,285,2002
mutation in microorganisms Salmonella typhimurium 108,000 ng/plate/72 hour (+enzymatic activation step) MUREAV 542,49,2003
mutation in microorganisms Salmonella typhimurium 0.1 µmol/plate/72 hour (-enzymatic activation step) MUREAV 558,93,2004
mutation in microorganisms Salmonella typhimurium 20 µg/plate/48 hour TCMUD8 23,85,2003
micronucleus test intraperitoneal
mouse 		1 gm/kg EMMUEG 14,207,1989
gene conversion and mitotic recombination Saccharomyes cerevisiae 500 mg/L MUREAV 78,243,1980
mutation in mammalian somatic cells mouse lymphocyte 50 mg/L JEPTDQ 1(1),87,1977
morphological transform hamster embryo 500 µg/L NCIMAV 58,243,1981
morphological transform mouse embryo 10 µmol/L/24 hour NATUAS 260,368,1976
morphological transform mouse other cell types 10 µmol/L NATUAS 260,368,1976
morphological transform rat embryo 80 µg/plate JJATDK 1,190,1981
other mutation test systems oral
mouse 		250 mg/kg MUREAV 343,157,1995
other mutation test systems Salmonella typhimurium 5 mg/L MUREAV 192,239,1987
phage inhibition capacity Escherichia coli 400 µg/plate MGBUA3 41,3,1976
sister chromatid exchange hamster ovary 25 mg/L MUREAV 56,207,1977
sex chromosome loss and nondisjunction parenteral
Drosophila melanogaster 		5 mmol/L MUREAV 56,31,1977
sex chromosome loss and nondisjunction oral
Drosophila melanogaster 		1,200 µmol/L/3 day MUREAV 48,181,1977
specific locus test oral
Drosophila melanogaster 		2 mmol/L MUREAV 341,161,1995


REPRODUCTIVE EFFECTS DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
intraperitoneal
rat 		lowest published toxic dose: 3 gm/kg (30 day male) Reproductive: Effects on embryo or fetus: Fetal death SheCW# 25MAR1977
subcutaneous
mouse 		lowest published toxic dose: 2,560 mg/kg (6-15 day pregnant) Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus)

Reproductive: Specific developmental abnormalities: Craniofacial (including nose and tongue)

Reproductive: Specific developmental abnormalities: Cardiovascular (circulatory) system 		TJADAB 19,37A,1979
subcutaneous
mouse 		lowest published toxic dose: 7,680 mg/kg (6-15 day pregnant) Reproductive: Effects on fertility: Litter size (e.g., # fetuses per litter; measured before birth) TJADAB 24,253,1981


TUMORIGENIC DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
N/R
 N/R N/R N/R


ACUTE TOXICITY DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
intraperitoneal
rat 		lethal dose (50 percent kill): >1,600 mg/kg N/R JTEHD6 2,657,1977
oral
mouse 		lethal dose (50 percent kill): 681 mg/kg N/R NCILB* NCI-E-C-72-3252,1973
oral
rat 		lethal dose (50 percent kill): 681 mg/kg N/R NCILB* NCI-E-C-72-3252,1973


OTHER MULTIPLE DOSE DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
N/R
 N/R N/R N/R


REVIEWS:

ORGANIZATION STANDARD
REFERENCE
International Agency for Research on Cancer (IARC) Cancer Review Animal Inadequate Evidence IMEMDT 16,63,1978
International Agency for Research on Cancer (IARC) Cancer Review Human No Adequate Data IMEMDT 16,63,1978
International Agency for Research on Cancer (IARC) Cancer Review Group 3 IMSUDL 7,56,1987
TOXICOLOGY REVIEW
JACTDZ 4(3),161,1985


STANDARDS AND REGULATIONS:

ORGANIZATION STANDARD
REFERENCE
N/R N/R N/R


NIOSH DOCUMENTATION AND SURVEILLANCE:

ORGANIZATION STANDARD or SURVEY
REFERENCE
National Occupational Exposure Survey 1983 National Occupational Exposure Survey 1983: Hazard Code: A1601;
Number of Industries 1;
Total Number of Facilities 2,065;
Number of Occupations 1;
Total Number of Employees Exposed 17,652;
Total Number of Female Employees Exposed 11,624


STATUS IN FEDERAL AGENCIES:

ORGANIZATION
REFERENCE
EPA GENETOX PROGRAM 1988, Positive: Body fluid assay; SHE-clonal assay
EPA GENETOX PROGRAM 1988, Positive: Cell transform.-C3H/10T1/2
EPA GENETOX PROGRAM 1988, Positive: Cell transform.-RLV F344 rat embryo
EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Positive: D melanogaster Sex-linked lethal
EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro cytogenetics-nonhuman
EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro SCE-nonhuman
EPA GENETOX PROGRAM 1988, Negative: Carcinogenicity-mouse/rat; N crassa-aneuploidy
EPA GENETOX PROGRAM 1988, Negative: Sperm morphology-mouse; S cerevisiae-reversion
EPA GENETOX PROGRAM 1988, Inconclusive: Rodent dominant lethal; Mammalian micronucleus
EPA GENETOX PROGRAM 1988, Inconclusive: In vitro UDS-human fibroblast
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, FINAL RULE FEREAC 47,26992,82
Used in semi-permanent hair coloring products, and as reagent for alpha keto acids
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NCI Carcinogenesis Bioassay (feed);no evidence:mouse,rat NCITR* NCI-TR-180,1979


REFERENCES:

CODEN
REFERENCE
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut Sts., Philadelphia, PA 19106) V.1- 1941-
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House, Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.10- 1987-
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For publisher information, see EMMUEG.
FEREAC Federal Register. (U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402) V.1- 1936-
GMCRDC Gann Monograph on Cancer Research. (Plenum Pub. Corp., 233 Spring St., New York, NY 10013) No. 11- 1971-
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) V.1- 1972-
IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) No.1- 1979-
JACTDZ Journal of the American College of Toxicology. (Mary Ann Liebert, Inc., 1651 Third Ave., New York, NY 10128) V.1-12, 1982-1993. Discontinued.
JAPTO* Journal of Applied Toxicology (John Wiley & Sons, Ltd., Oldlands Way Bognor Regis West Sussex, PO22 9SA England) V.1- 1981-
JEPTDQ Journal of Environmental Pathology and Toxicology. (Park Forest South, IL) V.1-5(3), 1977-81(?). For publisher information, see JEPOEC.
JJATDK JAT, Journal of Applied Toxicology. (John Wiley & Sons Ltd., Baffins Lane, Chichester, W. Sussex PO19 1UD, UK) V.1- 1981-
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub., 1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
MGBUA3 Microbial Genetics Bulletin. (Ohio State Univ., College of Biological Science, Dept. of Microbiology, 484 W. 12th Ave., Columbus, OH 43210) No.1- 1950-
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964-
NATUAS Nature. (Nature Subscription Dept., POB 1018, Manasguan, NJ 08736) V.1- 1869-
NCILB* Progress Report for Contract No. NIH-NCI-E-C-72-3252, Submitted to the National Cancer Institute by Litton Bionetics, Inc. (Bethesda, MD)
NCIMAV National Cancer Institute, Monograph. (U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402) No.1- 1959-
NCITR* National Cancer Institute Carcinogenesis Technical Report Series. (Bethesda, MD) No.0-205. For publisher information, see NTPTR*.
SheCW# Personal Communication from C.W. Sheu, Genetic Toxicology Branch, FDA, Mar. 25, 1977
TCMUD8 Teratogenesis, Carcinogenesis, and Mutagenesis. (Alan R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1980-
TJADAB Teratology, The International Journal of Abnormal Development. (Alan R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1968-
VHTODE Veterinary and Human Toxicology. (American College of Veterinary and Comparative Toxicology, Publication Office, Comparative Toxicology, Manhattan, KS 66506) V.19- 1977-

Used in semi-permanent hair coloring products, and as reagent for alpha keto acids

RTECS Compound Description:
   Tumorigen
   Mutagen
   Reproductive Effector

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