The Registry of Toxic Effects of Chemical Substances

1,3 - Butadiene, hexachloro -

RTECS #: EJ0700000

CAS #: 87-68-3

UPDATE: November 2004 MW: 260.74 MF: C4Cl6


NOTE:

TABLE OF CONTENTS:
  1. SYNONYMS:
  2. SKIN AND EYE IRRITATION DATA:
  3. MUTATION DATA:
  4. REPRODUCTIVE EFFECTS DATA:
  5. TUMORIGENIC DATA:
  6. ACUTE TOXICITY DATA:
  7. OTHER MULTIPLE DOSE DATA:
  8. REVIEWS:
  9. STANDARDS AND REGULATIONS:
  10. NIOSH DOCUMENTATION AND SURVEILLANCE:
  11. STATUS IN FEDERAL AGENCIES:
  12. REFERENCES:

SYNONYMS:

  1. 1,1,2,3,4,4 - Hexachloro - 1,3 - butadiene
  2. 1,3 - Hexachlorobutadiene
  3. Dolen - pur
  4. GP - 40 - 66:120
  5. HCBD
  6. Hexachlor - 1,3 - butadien (Czech)
  1. Hexachlorbutadiene
  2. Hexachloro - 1,3 - butadiene
  3. Hexachlorobutadiene
  4. Hexachlorobutadiene (ACGIH)
  5. Perchlorobutadiene
  6. RCRA waste number U128


SKIN AND EYE IRRITATION DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
eye
rabbit 		162 mg mild EJTXAZ 9,171,1976
eye
rabbit 		500 mg/24 hour mild 85JCAE -,116,1986
skin
rabbit 		810 mg/24 hour moderate EJTXAZ 9,171,1976
skin
rabbit 		500 mg/24 hour mild 85JCAE -,116,1986


MUTATION DATA AND REFERENCES:

SYSTEM TEST ROUTE/
ORGANISM/
TISSUE 		DOSE REFERENCE
cytogenetic analysis human lymphocyte 1 mg/L CYGEDX 22(4),46,1988
cytogenetic analysis oral
mouse 		2 mg/kg CYGEDX 22(4),46,1988
cytogenetic analysis inhalation
mouse 		10 mg/m3 CYGEDX 22(4),46,1988
unscheduled DNA synthesis hamster embryo 2 mg/L CALEDQ 23,297,1984
unscheduled DNA synthesis oral
rat 		77 gm/kg/11 week TXAPA9 60,287,1981
mutation in microorganisms Salmonella typhimurium 1 mg/plate (+enzymatic activation step) DHEFDK FDA-78-1046,1978
mutation in microorganisms Salmonella typhimurium 320 µg/plate (-enzymatic activation step) CRNGDP 7,431,1986
morphological transform hamster embryo 10 mg/L CALEDQ 23,297,1984
sister chromatid exchange hamster ovary 1,400 µg/L EMMUEG 10(Suppl 10),1,1987


REPRODUCTIVE EFFECTS DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
inhalation
rat 		lowest published toxic concentration: 15 ppm/6 hour (6-20 day pregnant) Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus) TOLED5 47,235,1989
intraperitoneal
rat 		lowest published toxic dose: 150 mg/kg (1-15 day pregnant) Reproductive: Effects on fertility: Post- implantation mortality (e.g., dead and/or resorbed implants per total number of implants)

Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus) 		SWEHDO 7(Suppl 4),66,1981
oral
mouse 		lowest published toxic dose: 10,920 µg/kg (13 week male) Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count) NTIS** PB91-185884
oral
rat 		lowest published toxic dose: 45 mg/kg (13 week male/13 week prior to copulation-3 week after birth) Reproductive: Effects on newborn: Biochemical and metabolic TXAPA9 42,387,1977
oral
rat 		lowest published toxic dose: 4 gm/kg (13 week male/13 week prior to copulation-3 week after birth) Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain) TXAPA9 42,387,1977
oral
rat 		lowest published toxic dose: 178 mg/kg (1-22 day pregnant) Reproductive: Specific developmental abnormalities: Central nervous system

Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain) 		AAGEAA 89(8),44,1985
oral
rat 		lowest published toxic dose: 20 mg/kg (multigenerations) Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus) NTIS** #PB93-123867
oral
rat 		lowest published toxic dose: 75 mg/kg (multigenerations) Reproductive: Specific developmental abnormalities: Urogenital system

Reproductive: Effects on newborn: Biochemical and metabolic 		NTIS** #PB93-123867
oral
rat 		lowest published toxic dose: 225 mg/kg (17-22 day pregnant) Reproductive: Effects on newborn: Live birth index (Litter size (e.g., # fetuses per litter; measured after birth) NTIS** #PB93-123867
oral
rat 		lowest published toxic dose: 37.5 mg/kg (multigenerations) Reproductive: Effects on newborn: Viability index (e.g., # alive at day 4 per # born alive)

Reproductive: Effects on newborn: Biochemical and metabolic 		NTIS** #PB93-123867
oral
rat 		lowest published toxic dose: 5 mg/kg (multigenerations) Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain) NTIS** #PB93-123867
subcutaneous
rat 		lowest published toxic dose: 20 mg/kg (1 day prior to copulation) Reproductive: Effects on newborn: Weaning or lactation index (e.g., # alive at weaning per # alive at day 4) GISAAA 31,33,1966


TUMORIGENIC DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
oral
rat 		lowest published toxic dose: 15 gm/kg/2 year- continuous Tumorigenic: Carcinogenic by RTECS criteria

Kidney, Ureter, and Bladder: Kidney tumors 		AIHAAP 38,589,1977


ACUTE TOXICITY DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
inhalation
mammal (species unspecified) 		lethal concentration (50 percent kill): 370 mg/m3 N/R GISAAA 55(5),72,1990
inhalation
mouse 		lethal concentration (50 percent kill): 370 mg/m3 N/R GISAAA 37(2),32,1972
inhalation
mouse 		lethal concentration (50 percent kill): 370 mg/m3/2 hour N/R VCVGH* -,518,1990
inhalation
rat 		lethal concentration (50 percent kill): 630 mg/m3/4 hour N/R VCVGH* -,518,1990
intraperitoneal
mouse 		lethal dose (50 percent kill): 76 mg/kg N/R EJTXAZ 7,247,1974
intraperitoneal
mouse 		lowest published toxic dose: 25 mg/kg Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) VCVGH* -,519,1990
intraperitoneal
rat 		lethal dose (50 percent kill): 175 mg/kg Behavioral: Somnolence (general depressed activity)

Behavioral: Irritability 		EJTXAZ 8,180,1975
intraperitoneal
rat 		lowest published toxic dose: 50 mg/kg Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) VCVGH* -,519,1990
intraperitoneal
rat 		lowest published toxic dose: 300 mg/kg Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)

Kidney, Ureter, and Bladder: Other changes in urine composition 		VCVGH* -,519,1990
oral
cat 		lethal dose (50 percent kill): 187 mg/kg N/R VCVGH* -,519,1990
oral
guinea pig 		lethal dose (50 percent kill): 90 mg/kg N/R VCVGH* -,519,1990
oral
guinea pig 		lowest published toxic dose: 10 mg/kg Nutritional and Gross Metabolic: Conditioned vitamin deficiency VCVGH* -,519,1990
oral
hamster 		lethal dose (50 percent kill): 960 mg/kg N/R TXAPA9 48,A192,1979
oral
mouse 		lethal dose (50 percent kill): 51 mg/kg N/R VCVGH* -,518,1990
oral
rat 		lethal dose (50 percent kill): 82 mg/kg N/R GISAAA 37(2),32,1972
oral
rat 		lowest published toxic dose: 10 mg/kg Nutritional and Gross Metabolic: Conditioned vitamin deficiency VCVGH* -,519,1990
oral
rat 		lowest published toxic dose: 8.1 mg/kg Spinal Cord: Other degenerative changes VCVGH* -,520,1990
skin
mouse 		lethal dose (50 percent kill): 150 mg/kg N/R VCVGH* -,518,1990
skin
rat 		lethal dose (50 percent kill): 4,500 mg/kg N/R VCVGH* -,519,1990
skin
rabbit 		lethal dose (50 percent kill): 100 mg/kg N/R GISAAA 37(2),32,1972
unreported route
mammal (species unspecified) 		lethal dose (50 percent kill): 200 mg/kg N/R 30ZDA9 -,40,1971


OTHER MULTIPLE DOSE DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
inhalation
man 		lowest published toxic concentration: 0.2 ppm/9 year- intermittent Liver: Change in gall bladder structure or function

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol) 		BJIMAG 49,700,1992
inhalation
rat 		lowest published toxic concentration: 100 ppm/6 hour/2 week- intermittent Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)

Nutritional and Gross Metabolic: Weight loss or decreased weight gain

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field 		BJIMAG 27,1,1970
inhalation
rat 		lowest published toxic concentration: 26 mg/m3/2 hour/30 day- intermittent Blood: Other changes

Blood: Changes in leukocyte (WBC) count

Nutritional and Gross Metabolic: Weight loss or decreased weight gain 		GISAAA 37(2),32,1972
intraperitoneal
rat 		lowest published toxic dose: 313 mg/kg/3 day- intermittent Liver: Other changes

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Phosphatases 		TIHEEC 8,191,1992
oral
guinea pig 		lowest published toxic dose: 420 mg/kg/30 week- intermittent Liver: Other changes

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Immunological Including Allergic: Increase in cellular immune response 		GTPZAB 9(11),50,1965
oral
mouse 		lowest published toxic dose: 540 mg/kg/15 day- continuous Cardiac: Changes in heart weight

Endocrine: Changes in spleen weight

Nutritional and Gross Metabolic: Weight loss or decreased weight gain 		JEPOEC 9,323,1989
oral
mouse 		lowest published toxic dose: 1,092 mg/kg/13 week- continuous Cardiac: Changes in heart weight

Kidney, Ureter, and Bladder: Changes in bladder weight

Nutritional and Gross Metabolic: Weight loss or decreased weight gain 		NTIS** PB91-185884
oral
rat 		lowest published toxic dose: 1,274 mg/kg/26 week- intermittent Brain and Coverings: Other degenerative changes

Behavioral: Alteration of operant conditioning

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol) 		GTPZAB 11(3),23,1967
oral
rat 		lowest published toxic dose: 14,600 mg/kg/2 year- continuous Kidney, Ureter, and Bladder: Other changes in urine composition

Kidney, Ureter, and Bladder: Other changes

Nutritional and Gross Metabolic: Weight loss or decreased weight gain 		EVHPAZ 21,49,1977
oral
rat 		lowest published toxic dose: 105 mg/kg/14 day- continuous Kidney, Ureter, and Bladder: Changes in bladder weight

Nutritional and Gross Metabolic: Weight loss or decreased weight gain 		TXAPA9 47,1,1979
oral
rat 		lowest published toxic dose: 794 mg/kg/18 week- intermittent Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)

Kidney, Ureter, and Bladder: Changes in bladder weight

Liver: Changes in liver weight 		TXAPA9 47,1,1979
oral
rat 		lowest published toxic dose: 560 mg/kg/4 week- continuous Kidney, Ureter, and Bladder: Other changes in urine composition

Endocrine: Changes in adrenal weight

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases 		FCTOD7 31,125,1993
oral
rat 		lowest published toxic dose: 80 mg/kg/16 day- continuous Behavioral: Food intake (animal)

Kidney, Ureter, and Bladder: Other changes

Nutritional and Gross Metabolic: Weight loss or decreased weight gain 		NTIS** #PB93-123867
oral
rat 		lowest published toxic dose: 160 mg/kg/16 day- continuous Behavioral: Tremor

Behavioral: Muscle weakness 		NTIS** #PB93-123867
oral
rat 		lowest published toxic dose: 160 mg/kg/16 day- continuous Behavioral: Sleep NTIS** #PB93-123867
oral
rat 		lowest published toxic dose: 160 mg/kg/16 day- continuous Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)

Kidney, Ureter, and Bladder: Changes in kidney weight 		NTIS** #PB93-123867
oral
rat 		lowest published toxic dose: 1,040 mg/kg/16 day- continuous Kidney, Ureter, and Bladder: Other changes in urine composition NTIS** #PB93-123867
oral
rat 		lowest published toxic dose: 320 mg/kg/16 day- continuous Behavioral: Fluid intake NTIS** #PB93-123867
oral
rat 		lowest published toxic dose: 600 mg/kg/16 day- continuous Kidney, Ureter, and Bladder: Urine volume increased NTIS** #PB93-123867
oral
rat 		lowest published toxic dose: 385 mg/kg/7 day- continuous Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field NTIS** #PB93-123867


REVIEWS:

ORGANIZATION STANDARD
REFERENCE
American Conference of Governmental Industrial Hygienists (ACGIH) Threshold Limit Value time-weighted average 0.02 ppm (skin) DTLVS* TLV/BEI,2003
American Conference of Governmental Industrial Hygienists (ACGIH) Threshold Limit Value Confirmed Animal Carcinogen with Unknown DTLVS* TLV/BEI,2003
International Agency for Research on Cancer (IARC) Cancer Review Animal Limited Evidence IMEMDT 20,179,1979
International Agency for Research on Cancer (IARC) Cancer Review Animal Limited Evidence IMEMDT 73,277,1999
International Agency for Research on Cancer (IARC) Cancer Review Human No Adequate Data IMEMDT 20,179,1979
International Agency for Research on Cancer (IARC) Cancer Review Human Inadequate Evidence IMEMDT 73,277,1999
International Agency for Research on Cancer (IARC) Cancer Review Group 3 IMEMDT 73,277,1999


STANDARDS AND REGULATIONS:

ORGANIZATION STANDARD
REFERENCE
Occupational Exposure Limit - AUSTRALIA time-weighted average 0.02 ppm (0.24 mg/m3), Skin, Carcinogen, JAN1993
Occupational Exposure Limit - AUSTRIA Skin, Suspected Carcinogen, JAN1999
Occupational Exposure Limit - BELGIUM time-weighted average 0.02 ppm (0.21 mg/m3), Skin, Carcinogen, JAN1993
Occupational Exposure Limit - FINLAND Carcinogen, JAN1999
Occupational Exposure Limit - GERMANY Skin, Carcinogen, JAN1999
Occupational Exposure Limit - NORWAY time-weighted average 0.02 ppm (0.24 mg/m3), JAN1999
Occupational Exposure Limit - THE NETHERLANDS MAC-TGG 0.24 mg/m3, 2003
Occupational Exposure Limit - SWITZERLAND MAK- week 0.02 ppm (0.24 mg/m3), Skin, JAN1999
Occupational Exposure Limit IN ARGENTINA, BULGARIA, COLOMBIA, JORDAN, KOREA American Conference of Governmental Industrial Hygienists (ACGIH) Threshold Limit Value;

Confirmed Animal Carcinogen with Unknown
Occupational Exposure Limit IN NEW ZEALAND, SINGAPORE, VIETNAM American Conference of Governmental Industrial Hygienists (ACGIH) Threshold Limit Value
Confirmed Animal Carcinogen with Unknown


NIOSH DOCUMENTATION AND SURVEILLANCE:

ORGANIZATION STANDARD or SURVEY
REFERENCE
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO HEXACHLOROBUTADIENE-air Carcinogen;
10 hour time-weighted average 0.02 ppm (Sk) 		NIOSH* DHHS #92-100,1992
National Occupational Exposure Survey 1983 National Occupational Exposure Survey 1983: Hazard Code: X6278;
Number of Industries 1;
Total Number of Facilities 3;
Number of Occupations 11;
Total Number of Employees Exposed 1,010;
Total Number of Female Employees Exposed 26


STATUS IN FEDERAL AGENCIES:

ORGANIZATION
REFERENCE
ATSDR TOXICOLOGY PROFILE (NTIS** PB/95/100160/AS)
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, FINAL RULE FEREAC 47,26992,82
Used as solvent, heat-transfer fluid, transformer, hydraulic fluid and wash liquor
EPA TSCA Section 8(d) unpublished health/safety studies
On EPA IRIS database
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NIOSH Analytical Method, 1994: Hexachlorobutadiene, 2543
NTP Toxicity studies, RPT# TOX-01, October 2000


REFERENCES:

CODEN
REFERENCE
30ZDA9 "Chemistry of Pesticides," Melnikov, N.N., New York, Springer-Verlag New York, Inc., 1971
85JCAE "Prehled Prumyslove Toxikologie; Organicke Latky," Marhold, J., Prague, Czechoslovakia, Avicenum, 1986
AAGEAA Arkhiv Anatomii, Gistologii i Embriologii. Archives of Anatomy, Histology and Embryology. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1-101, 1916-91. Discontinued
AIHAAP American Industrial Hygiene Association Journal. (AIHA, 475 Wolf Ledges Pkwy., Akron, OH 44311) V.19- 1958-
BJIMAG British Journal of Industrial Medicine. (British Medical Journal, Box 560B, Kennebunkport, ME 04046) V.1- 1944-
CALEDQ Cancer Letters (Shannon, Ireland). (Elsevier Scientific Pub. Ireland Ltd., POB 85, Limerick, Ireland) V.1- 1975-
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House, Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
CYGEDX Cytology and Genetics (English Translation). Translation of TGANAK. (Allerton Press Inc., 150 Fifth Ave., New York, NY 10011) V.8- 1974-
DHEFDK HEW Publication (FDA. United States). (Washington, DC) ?-1979(?). For publisher information, see HPFSDS.
DTLVS* The Threshold Limit Values (TLVs) and Biological Exposure Indices (BEIs) booklet issues by American Conference of Governmental Industrial Hygienists (ACGIH), Cincinnati, OH, 1996
EJTXAZ European Journal of Toxicology and Environmental Hygiene. (Paris, France) V.7-9, 1974-76. For publisher information, see TOERD9.
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.10- 1987-
EVHPAZ EHP, Environmental Health Perspectives. (U.S. Government Printing Office, Supt of Documents, Washington, DC 20402) No.1- 1972-
FCTOD7 Food and Chemical Toxicology. (Pergamon Press Inc., Maxwell House, Fairview Park, Elmsford, NY 10523) V.20- 1982-
FEREAC Federal Register. (U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402) V.1- 1936-
GISAAA Gigiena i Sanitariya. For English translation, see HYSAAV. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1- 1936-
GTPZAB Gigiena Truda i Professional'nye Zabolevaniya. Labor Hygiene and Occupational Diseases. (V/O Mezhdunarodnaya Kniga, 113095 Moscow, USSR) V.1-36, 1957-1992. For publisher information, see MTPEEI
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) V.1- 1972-
JEPOEC Journal of Environmental Pathology, Toxicology and Oncology. (Chem-Orbital, POB 134, Park Forest, IL 60466) V.5(4)- 1984-
NIOSH* National Institute of Occupational Safety and Health, U.S. Dept. of Health, Education, and Welfare, Reports and Memoranda.
NTIS** National Technical Information Service. (Springfield, VA 22161) Formerly U.S. Clearinghouse for Scientific & Technical Information.
SWEHDO Scandinavian Journal of Work, Environment and Health. (Haartmaninkatu 1, SF-00290 Helsinki, 29, Finland) V.1- 1975-
TIHEEC Toxicology and Industrial Health. (Princeton Scientific Pub. Co., POB 2155, Princeton, NJ 08540) V.1- 1985-
TOLED5 Toxicology Letters. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1977-
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959-
VCVGH* "Vrednie chemichescie veshestva, galogenproisvodnie uglevodorodov". (Hazardous substances: Galogenated hydrocarbons) Bandman A.L. et al., Chimia, 1990.

Used as solvent, heat-transfer fluid, transformer, hydraulic fluid and wash liquor

NIOSH PROFILE (HEXACHLOROBUTADIENE), SRI, 2/77

RTECS Compound Description:
   Agricultural Chemical
   Tumorigen
   Mutagen
   Reproductive Effector
   Human Data
   Primary Irritant

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