The Registry of Toxic Effects of Chemical Substances

Benzene, m - dinitro -

RTECS #: CZ7350000

CAS #: 99-65-0

UPDATE: February 2005 MW: 168.12 MF: C6H4N2O4


NOTE:

TABLE OF CONTENTS:
  1. SYNONYMS:
  2. SKIN AND EYE IRRITATION DATA:
  3. MUTATION DATA:
  4. REPRODUCTIVE EFFECTS DATA:
  5. ACUTE TOXICITY DATA:
  6. OTHER MULTIPLE DOSE DATA:
  7. REVIEWS:
  8. STANDARDS AND REGULATIONS:
  9. NIOSH DOCUMENTATION AND SURVEILLANCE:
  10. STATUS IN FEDERAL AGENCIES:
  11. REFERENCES:

SYNONYMS:

  1. Benzene, 1,3 - dinitro -
  2. Binitrobenzene
  3. m - Dinitrobenzene
  4. m - Dinitrobenzene (ACGIH:OSHA)
  1. 1,3 - Dinitrobenzene
  2. 2,4 - Dinitrobenzene
  3. 1,3 - Dinitrobenzol
  4. Dwunitrobenzen (Polish)


SKIN AND EYE IRRITATION DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
eye
rabbit 		100 mg N/R ATDAEI 1,168,1992


MUTATION DATA AND REFERENCES:

SYSTEM TEST ROUTE/
ORGANISM/
TISSUE 		DOSE REFERENCE
mutation in microorganisms Salmonella typhimurium 3,300 ng/plate (+enzymatic activation step) ENMUDM 2,531,1980
mutation in microorganisms Salmonella typhimurium 50 µg/plate (-enzymatic activation step) MUREAV 44,9,1977
other mutation test systems Salmonella typhimurium 12,500 µg/L MUREAV 272,91,1992


REPRODUCTIVE EFFECTS DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
intraperitoneal
rat 		lowest published toxic dose: 10 µg/kg (1 day male) Reproductive: Other effects on male REPTED 14,45,2000
oral
mouse 		lowest published toxic dose: 48 mg/kg (1 day male) Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Reproductive: Paternal effects: Testes, epididymis, sperm duct 		JTEHD6 28,67,1989
oral
rat 		lowest published toxic dose: 24 mg/kg (1 day male) Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Reproductive: Paternal effects: Testes, epididymis, sperm duct 		FAATDF 14,284,1990
oral
rat 		lowest published toxic dose: 33,600 µg/kg (16 week prior to copulation) Reproductive: Maternal effects: Ovaries, fallopian tubes JTEHD6 7,829,1981
oral
rat 		lowest published toxic dose: 48 mg/kg (1 day male) Reproductive: Effects on fertility: Male fertility index (e.g., # males impregnating females per # males exposed to fertile nonpregnant females) FAATDF 14,284,1990
oral
rat 		lowest published toxic dose: 90 mg/kg (12 week male) Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count) JTEHD6 19,477,1986


TUMORIGENIC DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
N/R
 N/R N/R N/R


ACUTE TOXICITY DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
intraperitoneal
mouse 		lowest published lethal dose: 200 mg/kg N/R JAPMA8 48,419,1959
intraperitoneal
rat 		lethal dose (50 percent kill): 28 mg/kg N/R AEPPAE 207,446,1949
intravenous
dog 		lethal dose (50 percent kill): 10 mg/kg Peripheral Nerve and Sensation: Spastic paralysis with/without sensory change

Behavioral: Convulsions or effect on seizure threshold

Blood: Methemoglobinemia- Carboxhemoglobinemia 		NTIS** AD-A066-307
oral
wild bird 		lethal dose (50 percent kill): 42 mg/kg N/R TXAPA9 21,315,1972
oral
cat 		lowest published lethal dose: 27 mg/kg Behavioral: Coma

Lung, Thorax, or Respiration: Cyanosis

Blood: Other hemolysis with or without anemia 		LANCAO 2,582,1901
oral
dog 		lowest published lethal dose: 600 mg/kg N/R NTIS** AD-A066-307
oral
human 		lowest published lethal dose: 28 mg/kg N/R 34ZIAG -,226,1969
oral
mouse 		lethal dose (50 percent kill): 74,700 µg/kg Behavioral: Somnolence (general depressed activity)

Behavioral: Tremor

Lung, Thorax, or Respiration: Respiratory stimulation 		ATDAEI 1,168,1992
oral
rat 		lethal dose (50 percent kill): 59,500 µg/kg Behavioral: Somnolence (general depressed activity)

Lung, Thorax, or Respiration: Dyspnea

Skin and Appendages: Other: Hair 		ATDAEI 1,168,1992
oral
rat 		lowest published toxic dose: 15 mg/kg Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Reproductive: Paternal effects: Testes, epididymis, sperm duct 		TXAPA9 92,54,1988
oral
rat 		lowest published toxic dose: 50 mg/kg Blood: Changes in spleen

Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Related to Chronic Data: Changes in testicular weight 		TXAPA9 92,54,1988
oral
rat 		lowest published toxic dose: 30 mg/kg Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Related to Chronic Data: Changes in testicular weight 		ARTODN 66,435,1992
oral
rat 		lowest published toxic dose: 60 mg/kg Kidney, Ureter, and Bladder: Other changes in urine composition

Reproductive: Paternal effects: Testes, epididymis, sperm duct 		ARTODN 66,435,1992
oral
rat 		lowest published toxic dose: 32 mg/kg Endocrine: Androgenic

Reproductive: Paternal effects: Testes, epididymis, sperm duct

Related to Chronic Data: Changes in testicular weight 		TXAPA9 95,255,1988
oral
rabbit 		lowest published lethal dose: 400 mg/kg N/R NTIS** AD-A066-307
skin
man 		lowest published toxic dose: 4 mg/kg/2 day- intermittent Behavioral: Change in motor activity (specific assay)

Lung, Thorax, or Respiration: Cyanosis 		LANCAO 2,582,1901
skin
rabbit 		lethal dose (50 percent kill): 1,900 mg/kg N/R ATDAEI 1,168,1992


OTHER MULTIPLE DOSE DATA AND REFERENCES:

ROUTE/
ORGANISM 		DOSE
EFFECT

 REFERENCE
implant
rat 		lowest published toxic dose: 30 mg/kg/6 day- continuous Brain and Coverings: Other degenerative changes

Blood: Hemorrhage 		TXAPA9 161,267,1999
intraperitoneal
rat 		lowest published toxic dose: 30 mg/kg/4 day- intermittent Brain and Coverings: Other degenerative changes

Behavioral: Ataxia

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field 		NRTXDN 20,83,1999
oral
rat 		lowest published toxic dose: 156 mg/kg/90 day- intermittent Endocrine: Changes in spleen weight

Blood: Methemoglobinemia- Carboxhemoglobinemia 		TOXID9 30,200,1996
oral
rat 		lowest published toxic dose: 265 mg/kg/8 week- continuous Blood: Pigmented or nucleated red blood cells

Nutritional and Gross Metabolic: Weight loss or decreased weight gain

Related to Chronic Data: Changes in testicular weight 		JTEHD6 7,829,1981
oral
rat 		lowest published toxic dose: 296 mg/kg/16 week- continuous Endocrine: Changes in spleen weight

Nutritional and Gross Metabolic: Weight loss or decreased weight gain

Related to Chronic Data: Changes in testicular weight 		JTEHD6 7,829,1981
oral
rat 		lowest published toxic dose: 700 mg/kg/2 week- intermittent Reproductive: Paternal effects: Testes, epididymis, sperm duct

Related to Chronic Data: Changes in testicular weight 		JTSCDR 25,251,2000


REVIEWS:

ORGANIZATION STANDARD
REFERENCE
American Conference of Governmental Industrial Hygienists (ACGIH) Threshold Limit Value time-weighted average 0.15 ppm (skin) DTLVS* TLV/BEI,2002
TOXICOLOGY REVIEW
JTSCDR 25,1,2000
TOXICOLOGY REVIEW
TOLED5 140-141,105,2003


STANDARDS AND REGULATIONS:

ORGANIZATION STANDARD
REFERENCE
Mine Safety and Health Administration (MSHA) STANDARD - air time-weighted average 0.15 ppm (1 mg/m3) (skin) DTLVS* 3,92,1971
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (General Industry) 8 hour time-weighted average 1 mg/m3 (skin) CFRGBR 29,1910.1000,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Construction) 8 hour time-weighted average 1 mg/m3 (skin) CFRGBR 29,1926.55,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Shipyards) 8 hour time-weighted average 1 mg/m3 (skin) CFRGBR 29,1915.1000,1993
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Federal Contractors) 8 hour time-weighted average 1 mg/m3 (skin) CFRGBR 41,50-204.50,1994
Occupational Exposure Limit - AUSTRIA Skin, Suspected Carcinogen, JAN1999
Occupational Exposure Limit - DENMARK time-weighted average 0.15 ppm (1 mg/m3), Skin, JAN1999
Occupational Exposure Limit - FRANCE VME 0.15 ppm (1 mg/m3), JAN1999
Occupational Exposure Limit - JAPAN Occupational Exposure Limit 0.15 ppm (1 mg/m3), Skin, JAN1999
Occupational Exposure Limit - UNITED KINGDOM time-weighted average 0.15 ppm (1.0 mg/m3), short term exposure limit 0.5 ppm, Skin, SEP2000
Occupational Exposure Limit - THE NETHERLANDS MAC-TGG 1 mg/m3, 2003


NIOSH DOCUMENTATION AND SURVEILLANCE:

ORGANIZATION STANDARD or SURVEY
REFERENCE
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO m-DINITROBENZENE-air 10 hour time-weighted average 1 mg/m3 (Sk) NIOSH* DHHS #92-100,1992
National Occupational Hazard Survey 1974 National Occupational Hazard Survey 1974: Hazard Code: 27046;
Number of Industries 2;
Total Number of Facilities 184;
Number of Occupations 3;
Total Number of Employees Exposed 1,754
National Occupational Exposure Survey 1983 National Occupational Exposure Survey 1983: Hazard Code: 27046;
Number of Industries 2;
Total Number of Facilities 41;
Number of Occupations 4;
Total Number of Employees Exposed 2,489;
Total Number of Female Employees Exposed 1,914


STATUS IN FEDERAL AGENCIES:

ORGANIZATION
REFERENCE
EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test
EPA TSCA Section 8(b) CHEMICAL INVENTORY
On EPA IRIS database
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001


REFERENCES:

CODEN
REFERENCE
34ZIAG "Toxicology of Drugs and Chemicals," Deichmann, W.B., New York, Academic Press, Inc., 1969
AEPPAE Naunyn-Schmiedeberg's Archiv fuer Experimentelle Pathologie und Pharmakologie. (Berlin, Ger.) V.110-253, 1925-66. For publisher information, see NSAPCC.
ARTODN Archives of Toxicology. (Springer-Verlag, Heidelberger Pl. 3, D-1000 Berlin 33, Fed. Rep. Ger.) V.32- 1974-
ATDAEI Acute Toxicity Data. Journal of the American College of Toxicology, Part B. (Mary Ann Liebert, Inc., 1651 Third Ave., New York, NY 10128) V.1- 1990-
CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402)
DTLVS* The Threshold Limit Values (TLVs) and Biological Exposure Indices (BEIs) booklet issues by American Conference of Governmental Industrial Hygienists (ACGIH), Cincinnati, OH, 1996
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For publisher information, see EMMUEG.
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1-40, 1981-97. For publisher information, see TOSCF2
JAPMA8 Journal of the American Pharmaceutical Association, Scientific Edition. (Washington, DC) V.29-49, 1940-60. For publisher information, see JPMSAE.
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub., 1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
JTSCDR Journal of Toxicological Sciences. (Japanese Soc. of Toxicological Sciences, 4th Floor, Gakkai Center Bldg., 4-16, Yayoi 2-chome, Bunkyo-ku, Tokyo 113, Japan) V.1- 1976-
LANCAO Lancet. (7 Adam St., London WC2N 6AD, UK) V.1- 1823-
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964-
NIOSH* National Institute of Occupational Safety and Health, U.S. Dept. of Health, Education, and Welfare, Reports and Memoranda.
NRTXDN Neurotoxicology. (Intox Press, Inc., POB 34075, Little Rock, AR 72203) V.1- 1979-
NTIS** National Technical Information Service. (Springfield, VA 22161) Formerly U.S. Clearinghouse for Scientific & Technical Information.
REPTED Reproductive Toxicology. (Pergamon Press Inc., Maxwell House, Fairview Park, Elmsford, NY 10523) V.1- 1987-
TOLED5 Toxicology Letters. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1977-
TOXID9 Toxicologist. (Soc. of Toxicology, Inc., 475 Wolf Ledge Parkway, Akron, OH 44311) V.1- 1981-
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959-

NIOSH PROFILE (NITROBENZENES), SRC, 10/77

RTECS Compound Description:
   Mutagen
   Reproductive Effector
   Human Data
   Primary Irritant

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